mouse anti cse antibody  (Cell Signaling Technology Inc)

Journal: Antioxidants

Article Title: Role of Sulfide Quinone Oxidoreductase and Supersulfides in Hepatic Ischemia–Reperfusion Injury in Mice

doi: 10.3390/antiox15010094

Figure Lengend Snippet: Liver function and histological changes and level of sulfide quinone oxidoreductase (SQOR) in hepatic ischemia–reperfusion injury in mice. ( a ) Flowchart of the experimental protocols. ( b ) The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) 4 h after reperfusion in mice. ( c ) Representative images of hematoxylin and eosin (H&E) staining 4 h after reperfusion in mice. The scale bars are 50 μm in H&E images. ( d ) Upper part of the figure shows representative Western blotting images of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), sulfide quinone oxidoreductase (SQOR), ethylmalonic encephalopathy 1 (ETHE1), thiosulfate sulfurtransferase (TST), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein levels in liver extracts from sham and IRI mice. Lower part of the figure shows differences in CBS/GAPDH, CSE/GAPDH, SQOR/GAPDH, ETHE1/GAPDH, and TST/GAPDH in each group. The data are presented as n = 6 per group. Significant differences between groups were determined using one-way analysis of variance. Data are expressed as the means ± standard deviations, **** p < 0.0001.

Article Snippet: After transfer, the membranes were blocked with Every blocking buffer (Bio-Rad Laboratories, Inc.) and immunoblotted with one of the primary antibodies at 4 °C overnight: mouse anti-GAPDH antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA, Cat# 5174), mouse anti-CSE antibody (1:1000; Cell Signaling Technology, Cat#19689), mouse anti-CBS antibody (1:1000; Cell Signaling Technology, Cat# 14782S), mouse anti-SQOR antibody (1:500; Cell Signaling Technology, Cat#17256-1-AP), mouse anti-TST antibody (1:1000; Gene Tex Company, Cat# GTX114858), and mouse anti-ETHE1 antibody (1:1000; Proteintech Group, Inc., Cat# 27786-1AP).

Techniques: Staining, Western Blot

Journal: Antioxidants

Article Title: Role of Sulfide Quinone Oxidoreductase and Supersulfides in Hepatic Ischemia–Reperfusion Injury in Mice

doi: 10.3390/antiox15010094

Figure Lengend Snippet: Relative protein levels of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), ethylmalonic encephalopathy 1 (ETHE1), and thiosulfate sulfurtransferase (TST) in the livers of AAV-control and AAV-shSQOR and AAV-shSQOR+Na 2 S 3 mice. Representative Western blotting images of CBS, CSE, ETHE1, TST, and GAPDH protein levels in liver extracts from each group of mice and differences in CBS/GAPDH, CSE/GAPDH, ETHE1/GAPDH, and TST/GAPDH in each group. The data are presented as n = 6 per group. Significant differences between groups were determined using one-way analysis of variance. Data are expressed as the means ± standard deviations, * p < 0.05, *** p < 0.001, and **** p < 0.0001.

Article Snippet: After transfer, the membranes were blocked with Every blocking buffer (Bio-Rad Laboratories, Inc.) and immunoblotted with one of the primary antibodies at 4 °C overnight: mouse anti-GAPDH antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA, Cat# 5174), mouse anti-CSE antibody (1:1000; Cell Signaling Technology, Cat#19689), mouse anti-CBS antibody (1:1000; Cell Signaling Technology, Cat# 14782S), mouse anti-SQOR antibody (1:500; Cell Signaling Technology, Cat#17256-1-AP), mouse anti-TST antibody (1:1000; Gene Tex Company, Cat# GTX114858), and mouse anti-ETHE1 antibody (1:1000; Proteintech Group, Inc., Cat# 27786-1AP).

Techniques: Control, Western Blot

Journal: Pharmacological research

Article Title: Hydrogen sulfide regulates the redox state of soluble guanylate cyclase in CSE -/- mice corpus cavernosum microcirculation.

doi: 10.1016/j.phrs.2023.106834

Figure Lengend Snippet: Fig. 2. CSE ablation reduces NO bioavail ability in the corpus cavernosum. A) The relaxant response induced by acetylcholine is significantly reduced in CSE-/- mice compared to the control (n = 10 mice). B) NOx production is significantly reduced in CSE-/- mice compared to control (n = 5 mice). Averaged densitometric protein expression of C) total eNOS and D) p- eNOS E) ratio of activated p-eNOS to total eNOS form (n = 5 mice). The p-eNOS and the ratio of p-eNOS/eNOS are significantly reduced in the CC of CSE-/- mice. Results are means ± SEM. * Indicates p < 0.05, * * p < 0.01, * **p < 0.001 vs control using a t-test or two- way ANOVA test followed by a Bonferroni post hoc test.

Article Snippet: Olivencia et al. Pharmacological Research 194 (2023) 106834 phosphate-buffered saline (PBS) containing 0.1% v/v Tween 20% and 3% nonfat dried milk (Applichem, Darmstadt, Germany) for one hour at room temperature and then incubated overnight at 4 ◦C with primary rabbit antibodies against CBS (1:1000; sc-67154, Santa Cruz Biotechnology, Heidelberg, Germany), 3MST (1:1000; NBP1–54734, Novus Biologicals, Abingdon, UK), p-eNOS (1:1000, 9571 s, Cell Signaling, Milan, Italy), p-PDE5 (1:500, PPD5–140AP, FabGennixInc, Frisco, USA), PDE5 (1:500; sc-32884, Santa Cruz Biotechnology, Heidelberg, Germany), sGC β1 subunit (1:1000; G4405, Merck, Rome, Italy), CYB5R3 (1:1000; 10894–1-AP, Proteintech, Manchester, UK), or mouse antibodies against CSE (1:1000; 60234–1-Ig, Proteintech, Manchester, UK), eNOS (1:1000; 610297, BD Transduction Laboratories, Milan, Italy).

Techniques: Control, Expressing